J Clin Endocrinol Metab. 2016 Dec 15:jc20163008. [Epub ahead of print]<\/p>\n
B\u00f8llehuus Hansen L(1,)(2,)(3), Rehfeld A(1,)(2,)(4), de Neergaard R(1,)(2),<\/p>\n
Nielsen JE(1,)(2), Iversen LH(5), Boisen IM(1,)(2,)(3), Mortensen LJ(1,)(2,)(3),<\/p>\n
Lanske B(3), Almstrup K(1,)(2), Carlsen E(5), Berg AH(6), J\u00f8rgensen N(1),<\/p>\n
Andersen AN(5), Juul A(1,)(2), Blomberg Jensen M(1,)(2,)(3).<\/p>\n
Author information:<\/p>\n
(1)a Department of Growth and Reproduction, Rigshospitalet, University of<\/p>\n
Copenhagen, Denmark. (2)b International Research and Research Training Centre in<\/p>\n
Endocrine Disruption of Male Reproduction and Child Health (EDMaRC),<\/p>\n
Rigshospitalet, University of Copenhagen, Denmark. (3)c Division of Bone and<\/p>\n
Mineral Research, Harvard School of Dental Medicine, Boston MA, US. (4)d<\/p>\n
Department of Cellular and Molecular Medicine, Faculty of Health Sciences,<\/p>\n
University of Copenhagen, Denmark. (5)e Fertility Clinic, Rigshospitalet,<\/p>\n
University of Copenhagen, Denmark. (6)f Department of Pathology, Beth Israel<\/p>\n
Deaconess Medical Center and Harvard Medical School, Boston, MA, US.<\/p>\n
CONTEXTO: El receptor de Vitamina D (VDR) y las enzimas implicadas en su activaci\u00f3n (CYP2R1, CYP27B1) e inactivaci\u00f3n (CYP24A1) se expresan en ovarios, test\u00edculos y espermatozoides.<\/p>\n
OBJETIVO: Determinar la respuesta a 1,25-dihidroxi vitamina D (1,25(OH)2D3) en espermatozoides de hombres controles y hombres inf\u00e9rtiles, identificar su exposici\u00f3n y c\u00f3mo 1,25(OH)2D3 afecta a la funci\u00f3n seminal.<\/p>\n
DISE\u00d1O: La expresi\u00f3n de espermatozoides de VDR, CYP2R1, CYP27B1, y CYP24A1 se analiz\u00f3 en hombres controles y hombres inf\u00e9rtiles. \u00a0Se determin\u00f3 la concentraci\u00f3n s\u00e9rica, la concentraci\u00f3n en l\u00edquido seminal, secreciones cervicales y l\u00edquido folicular ov\u00e1rico de 25-hydroxi vitamina D (25-OHD), 24,25-dihidroxi vitamina D (24,25(OH)2D3), y 1,25(OH)2D3. En espermatozoides se analiz\u00f3 el 1,25(OH)2D3.<\/p>\n
ENTORNO: Centro terciario de fertilidad.<\/p>\n
PARTICIPANTES: La expresi\u00f3n de prote\u00ednas en espermatozoides y an\u00e1lisis de la calidad seminal se llev\u00f3 a cabo en 230 hombres inf\u00e9rtiles y 114 controles. Los metabolitos de la Vit D se determinaron en fluidos de 245 hombres y 13 mujeres, mientras se emplearon 74 oocitos y 17 muestras de semen de donante para los tests de funci\u00f3n seminal.<\/p>\n
PRINCIPALES MEDIDAS: Expresi\u00f3n de VDR y CYP24A1 en fluido esperm\u00e1tico, concentraciones de 25-OHD, 24,25(OH)2D3 and 1,25(OH)2D3, efectos sobre la concentraci\u00f3n de calcio intracelular inducido por 1,25(OH)2D3, y uni\u00f3n esperma-oocito in vitro.<\/p>\n
RESULTDOS: VDR y CYP24A1 se expresaron en m\u00e1s del doble en los espermatozoides de hombres controles comparados con hombres sanos (p<0.01). Las concentraciones de 25-OHD, 24,25(OH)2D y 1,25(OH)2D3 fueron indetectables en fluido seminal pero fueron elevadas en fluido ov\u00e1rico folicular. Las concentraciones foliculares de 1,25(OH)2D3 indujeron un modesto incremento de calcio intracelular y de la uni\u00f3n esperma-oocito in vitro (p<0.05).<\/p>\n
CONCLUSION: La presencia de VDF y CYP24A1 principalmente en los espermatozoides de alta calidad apunta a que el 1,25(OH)2D3 disponible en el tracto reproductivo femenino podr\u00eda promocionar la selecci\u00f3n de los mejores gametos para la fertilizaci\u00f3n.<\/p>\n
DOI: 10.1210\/jc.2016-3008<\/p>\n
PMID: 27977320 \u00a0[PubMed - as supplied by publisher]<\/p>\n
Comentario: un nuevo ejemplo de la importancia de la vitamina D en la androlog\u00eda. Esta mol\u00e9cula parece estar implicada de manera capital en distintas entidades de la androlog\u00eda y merecer\u00e1 en el futuro m\u00e1s atenci\u00f3n.<\/p>\n
Eduard Garc\u00eda-Cruz<\/p>\n
Ur\u00f3logo. FECSM<\/p>\n
Urolog\u00eda y Salud del Hombre<\/p>\n
Servicio de Urolog\u00eda<\/p>\n
Hospital Cl\u00ednic de Barcelona<\/p>\n
www.reisho.com<\/p>\n","protected":false},"excerpt":{"rendered":"
J Clin Endocrinol Metab. 2016 Dec 15:jc20163008. [Epub ahead of print] B\u00f8llehuus Hansen L(1,)(2,)(3), Rehfeld A(1,)(2,)(4), de Neergaard R(1,)(2), Nielsen JE(1,)(2), Iversen LH(5), Boisen IM(1,)(2,)(3), Mortensen LJ(1,)(2,)(3), Lanske B(3), Almstrup K(1,)(2), Carlsen E(5), Berg AH(6), J\u00f8rgensen N(1), Andersen AN(5), Juul A(1,)(2), Blomberg Jensen M(1,)(2,)(3). Author information: (1)a Department of Growth and Reproduction, Rigshospitalet, University of ...contin\u00faa leyendo \"La selecci\u00f3n de espermatozoides de alta calidad podr\u00eda ser promovida por la activaci\u00f3n de la vitamina D en mujeres\"<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"categories":[3,5,11],"tags":[],"class_list":{"0":"post-645","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-alertasurologia","7":"category-articulos-de-interes","8":"category-infertilidad","9":"h-entry","10":"hentry","11":"h-as-article"},"_links":{"self":[{"href":"https:\/\/gua.aeu.es\/index.php?rest_route=\/wp\/v2\/posts\/645","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gua.aeu.es\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gua.aeu.es\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gua.aeu.es\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/gua.aeu.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=645"}],"version-history":[{"count":1,"href":"https:\/\/gua.aeu.es\/index.php?rest_route=\/wp\/v2\/posts\/645\/revisions"}],"predecessor-version":[{"id":646,"href":"https:\/\/gua.aeu.es\/index.php?rest_route=\/wp\/v2\/posts\/645\/revisions\/646"}],"wp:attachment":[{"href":"https:\/\/gua.aeu.es\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=645"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gua.aeu.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=645"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gua.aeu.es\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=645"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}